Calendar

May
24
Mon
Dissertation Defense: Xing Di
May 24 @ 12:00 pm
Dissertation Defense: Xing Di

Note: This is a virtual presentation. Here is the link for where the presentation will be taking place.

Title: Deep Learning Based Face Image Synthesis

Abstract: Face image synthesis is an important problem in the biometrics and computer vision communities due to its applications in law enforcement and entertainment. In this thesis, we develop novel deep neural network models and associated loss functions for two face image synthesis problems, namely thermal to visible face synthesis and visual attribute to face synthesis.

In particular, for thermal to visible face synthesis, we propose a model which makes use of facial attributes to obtain better synthesis. We use attributes extracted from visible images to synthesize attribute-preserved visible images from thermal imagery. A pre-trained attribute predictor network is used to extract attributes from the visible image. Then, a novel multi-scale generator is proposed to synthesize the visible image from the thermal image guided by the extracted attributes. Finally, a pre-trained VGG-Face network is leveraged to extract features from the synthesized image and the input visible image for verification.

In addition, we propose another thermal to visible face synthesis method based on a self-attention generative adversarial network (SAGAN) which allows efficient attention-guided image synthesis. Rather than focusing only on synthesizing visible faces from thermal faces, we also propose to synthesize thermal faces from visible faces. Our intuition is based on the fact that thermal images also contain some discriminative information about the person for verification. Deep features from a pre-trained Convolutional Neural Network (CNN) are extracted from the original as well as the synthesized images. These features are then fused to generate a template which is then used for cross-modal face verification.

Regarding attribute to face image synthesis, we propose the Att2SK2Face model for face image synthesis from visual attributes via sketch. In this approach, we first synthesize a facial sketch corresponding to the visual attributes and then generate the face image based on the synthesized sketch. The proposed framework is based on a combination of two different Generative Adversarial Networks (GANs) – (1) a sketch generator network which synthesizes realistic sketch from the input attributes, and (2) a face generator network which synthesizes facial images from the synthesized sketch images with the help of facial attributes.

Finally, we propose another synthesis model, called Att2MFace, which can simultaneously synthesize multimodal faces from visual attributes without requiring paired data in different domains for training the network. We introduce a novel generator with multimodal stretch-out modules to simultaneously synthesize multimodal face images. Additionally, multimodal stretch-in modules are introduced in the discriminator which discriminates between real and fake images.

Committee Members

  • Vishal Patel, Department of Electrical and Computer Engineering
  • Rama Chellappa, Department of Electrical and Computer Engineering
  • Carlos Castillo, Department of Electrical and Computer Engineering
May
25
Tue
Dissertation Defense: Arun Nair
May 25 @ 12:30 pm
Dissertation Defense: Arun Nair

Note: This is a virtual presentation. Here is the link for where the presentation will be taking place.

Title: Machine Learning for Beamforming in Ultrasound, Radar, and Audio

Abstract: Multi-sensor signal processing plays a crucial role in the working of several everyday technologies, from correctly understanding speech on smart home devices to ensuring aircraft fly safely. A specific type of multi-sensor signal processing called beamforming forms a central part of this thesis. Beamforming works by combining the information from several spatially distributed sensors to directionally filter information, boosting the signal from a certain direction but suppressing others. The idea of beamforming is key to the domains of ultrasound, radar, and audio.

Machine learning, succinctly defined by Tom Mitchell as “the study of algorithms that improve automatically through experience” is the other central part of this thesis. Machine learning, especially its sub-field of deep learning, has enabled breakneck progress in tackling several problems that were previously thought intractable. Today, machine learning powers many of the cutting edge systems we see on the internet for image classification, speech recognition, language translation, and more.

In this dissertation, we look at beamforming pipelines in ultrasound, radar, and audio from a machine learning lens and endeavor to improve different parts of the pipelines using ideas from machine learning. Starting off in the ultrasound domain, we use deep learning as an alternative to beamforming in ultrasound and improve the information extraction pipeline by simultaneously generating both a segmentation map and B-mode image of high quality directly from raw received ultrasound data.

Next, we move to the radar domain and study how deep learning can be used to improve signal quality in ultra-wideband synthetic aperture radar by suppressing radio frequency interference, random spectral gaps, and contiguous block spectral gaps. By training and applying the networks on raw single-aperture data prior to beamforming, it can work with myriad sensor geometries and different beamforming equations, a crucial requirement in synthetic aperture radar.

Finally, we move to the audio domain and derive a machine learning inspired beamformer to tackle the problem of ensuring the audio captured by a camera matches its visual content, a problem we term audiovisual zoom. Unlike prior work which is capable of only enhancing a few individual directions, our method enhances audio from a contiguous field of view.

Committee Members

  • Trac Tran, Department of Electrical and Computer Engineering
  • Muyinatu Bell, Department of Electrical and Computer Engineering
  • Vishal Patel, Department of Electrical and Computer Engineering
May
28
Fri
Dissertation Defense: Takeshi Uejima
May 28 @ 9:00 am
Dissertation Defense: Takeshi Uejima

Note: This is a virtual presentation. Here is the link for where the presentation will be taking place.

Title: A Unified Visual Saliency Model for Neuromorphic Implementation

Abstract: Although computer capabilities have expanded tremendously, a significant wall remains between the computer and the human brain. The brain can process massive amounts of information obtained from a complex environment and control the entire body in real time with low energy consumption. This thesis tackles this mystery by modeling and emulating how the brain processes information based on the available knowledge of biological and artificial intelligence as studied in neuroscience, cognitive science, computer science, and computer engineering.

Saliency modeling relates to visual sense and biological intelligence. The retina captures and sends much data about the environment to the brain. However, as the visual cortex cannot process all the information in detail at once, the early stages of visual processing discard unimportant information. Because only the fovea has high-resolution imaging, individuals move their eyeballs in the direction of the important part of the scene. Therefore, eye movements can be thought of as an observable output of the early visual process in the brain. Saliency modeling aims to understand this mechanism and predict eye fixations.

Researchers have built biologically plausible saliency models that emulate the biological process from the retina through the visual cortex. Although many saliency models have been proposed, most are not bio-realistic. This thesis models the biological mechanisms for the perception of texture, depth, and motion. While texture plays a vital role in the perception process, defining texture in a mathematical way is not easy. Thus, it is necessary to build an architecture of texture processing based on the biological perception mechanism. Binocular stereopsis is another intriguing function of the brain. While scholars have evaluated many computational algorithms for stereovision, pursuing biological plausibility means implementing a neuromorphic method into a saliency model. Motion is another critical clue that helps animals survive. In this thesis, the motion feature is implemented in a bio-realistic way based on neurophysiological observation.

Moreover, the thesis will integrate these processes and propose a unified saliency model that can handle 3D dynamic scenes in a similar way to how the brain deals with the real environment. Thus, this investigation will use saliency modeling to examine intriguing properties of human visual processing and discuss how the brain achieves this remarkable capability.

Committee Members

  • Ralph Etienne-Cummings, Department of Electrical and Computer Engineering
  • Andreas Andreou, Department of Electrical and Computer Engineering
  • Philippe Pouliquen, Department of Electrical and Computer Engineering
  • Ernst Niebur, Department of Neuroscience
Jun
29
Tue
Dissertation Defense: Yan Jiang
Jun 29 @ 1:00 pm
Dissertation Defense: Yan Jiang

Note: This is a virtual presentation. Here is the link for where the presentation will be taking place.

Title: Leveraging Inverter-Interfaced Energy Storage for Frequency Control in Low-Inertia Power Systems

Abstract: The shift from conventional synchronous generation to renewable inverter-interfaced sources has led to a noticeable degradation of frequency dynamics in power systems, mainly due to a loss of inertia. Fortunately, the recent technology advancement and cost reduction in energy storage facilitate the potential for higher renewable energy penetration via inverter-interfaced energy storage. With proper control laws imposed on inverters, the rapid power-frequency response from energy storage contributes to mitigating the degradation. A straightforward choice is to emulate the droop response and/or inertial response of synchronous generators through droop control (DC) or virtual inertia (VI), yet they do not necessarily fully exploit the benefits of inverter-interfaced energy storage. This thesis thus seeks to challenge this naive choice of mimicking synchronous generator characteristics by advocating for a principled control design perspective.

To achieve this goal, we build an analysis framework for quantifying the performance of power systems using signal and system norms, within which we perform a systematic study to evaluate the effect of different control laws on both frequency response metrics and storage economic metrics. More precisely, under a mild yet insightful proportionality assumption, we are able to perform a modal decomposition which allows us to get closed-form expressions or conditions for synchronous frequency, Nadir, rate of change of frequency (RoCoF), synchronization cost, frequency variance, and steady-state effort share. All of them pave the way for a better understanding of the sensitivities of various performance metrics to different control laws.

Our analysis unveils several limitations of traditional control laws, such as the inability of DC to improve the dynamic performance without sacrificing the steady-state performance and  the unbounded frequency variance introduced by VI in  the presence of frequency measurement noise. Therefore, rather than clinging to the idea of imitating synchronous generator behavior via inverter-interfaced energy storage, we prefer searching for better solutions.

We first propose dynam-i-c Droop control (iDroop)—inspired by the classical lead/lag compensator—which is proved to enjoy many good properties. First of all, the added degrees of freedom in iDroop allow to decouple the dynamic performance improvement from the steady-state performance. In addition, the lead/lag property of iDroop makes it less sensitive to stochastic power fluctuations and frequency measurement noise. Last but not least, iDroop can also be tuned either to achieve the zero synchronization cost or to achieve the Nadir elimination, by which we mean to remove the overshoot in the transient system frequency. Particularly, the Nadir elimination tuning of iDroop exhibits the potential for a balance among various performance metrics in reality. However, iDroop has no control over the RoCoF, which is undesirable in low-inertia power systems for the risk of falsely triggering protection.

We then propose frequency shaping control (FS)—an extension of iDroop—whose most outstanding feature is its ability to shape the system frequency dynamics following a sudden power imbalance into a first-order one with the specified synchronous frequency and RoCoF by adjusting two independent control parameters respectively.

We finally validate theoretical results through extensive numerical experiments performed on a more realistic power system test case that violates the proportionality assumption, which clearly confirms that our proposed control laws outperform the traditional ones in an overall sense.

Committee Members

  • Enrique Mallada, Department of Electrical and Computer Engineering
  • Pablo A. Iglesias, Department of Electrical and Computer Engineering
  • Dennice F. Gayme, Department of Mechanical Engineering
  • Petr Vorobev, Center for Energy Science and Technology, Skolkovo Institute of Science and Technology
Jun
30
Wed
Dissertation Defense: Ashwin Bellur
Jun 30 @ 10:00 am
Dissertation Defense: Ashwin Bellur

Note: This is a virtual presentation. Here is the link for where the presentation will be taking place.

Title: Bio-Mimetic Sensory Mapping with Attention for Auditory Scene Analysis

Abstract: The human auditory system performs complex auditory tasks such as having a conversation in a busy cafe or picking the melodic line of a particular instrument in an ensemble orchestra, with remarkable ease. The human auditory system also exhibits the ability to effortlessly adapt to constantly changing conditions and novel stimulus. The human auditory system achieves these through complex neuronal processes. First the low dimensional signal representing the acoustic stimulus is mapped to a higher dimensional space through a series of feed-forward neuronal transformations; wherein the different auditory objects in the scene are discernible. These feed-forward processes are then further complemented by the top-down processes like attention, driven by the cognitive regions to modulate the feed-forward processes in a manner that shines the spotlight on the object of interest; the interlocutor in the example of a busy cafe or the instrument of interest in the ensemble orchestra.

In this work, we explore leveraging these mechanisms observed in the mammalian brain, within computational frameworks, for addressing various auditory scene analysis tasks such as speech activity detection, environmental sound classification and source separation. We develop bio-mimetic computational strategies to model the feed-forward sensory mapping processes as well as the corresponding complementary top-down mechanisms capable of modulating the feed-forward processes during attention.

In the first part of this work, we show using Gabor filters as an approximation for the feed-forward processes, that retuning the feed-forward processes under top-down attentional feedback are extremely potent in enabling robust behavior in detecting speech activity. We introduce the notion of memory to represent prior knowledge of the acoustic objects and show that memories of objects can be used to deploy the necessary top-down feedback. Next, we expand the feed-forward processes using data-driven distributed deep belief system consisting of multiple streams to capture the stimulus from different spectrotemporal resolutions, a feature observed in the human auditory system. We show that such a distributed system with inherent redundancies, further complemented by top-down attentional mechanisms using distributed object memories allow for robust classification of environmental sounds in mismatched conditions. Finally, we show that incorporating the ideas of distributed processing and attentional mechanisms using deep neural networks leads to state-of-the-art performance for even complex tasks such as source separation. Further, we show that in such a distributed system, the sum of the parts are better than the individual parts and that this aspect can be used to generate real-time top-down feedback; which in turn can be used to adapt the network to novel conditions during inference.

Overall, the results of the work show that leveraging theses biologically inspired mechanisms within computational frameworks lead to enhanced robustness and adaptability to novel conditions, traits of the human auditory system that we sought to emulate.

Committee Members

Mounya Elhilali, Department of Electrical and Computer Engineering

Najim Dehak, Department of Electrical and Computer Engineering

Rama Chellappa, Department of Electrical and Computer Engineering

Dissertation Defense: Soohyun Lee
Jun 30 @ 2:00 pm
Dissertation Defense: Soohyun Lee

Note: This is a virtual presentation. Here is the link for where the presentation will be taking place.

Title: Optical coherence tomography (OCT) – guided ophthalmic therapy

Abstract: Optical coherence tomography (OCT), which provides cross-sectional images noninvasively with a micro-scale in real-time, has been widely applied for the diagnosis and treatment guidance for various ocular diseases.

In the first part of this work, we develop a hand-held subretinal-injector actively guided by a common-path OCT (CP-OCT) distal sensor. Subretinal injection is becoming increasingly prevalent in both scientific research and clinical communities as an efficient way of treating retinal diseases. It delivers drug or stem cells in the space between RPE and photoreceptor layers and, thus, directly affect resident cell and tissues in the subretinal space. However, the technique requires high stability and dexterity of surgeon due to fine anatomy of the retina, and it is challenging because of physiological motions of surgeons like hand tremor. We mainly focus on two aspects of the CP-OCT guided subretinal-injector: (i) A high-performance fiber probe based on high-index epoxy lensed-fiber to enhance the CP-OCT retinal image quality; (ii) Automated layer identification and tracking: Each retinal layer boundary, as well as retinal surface, is tracked using 1D convolutional neural network (CNN)-based segmentation on A-scans for accurate localization of a needle. The CNN model is integrated into the CP-OCT system for real-time target boundary distance sensing, and unwanted axial motions are compensated based on the target boundary tracking. The CP-OCT distal sensor guided system is tested on ex vivo bovine retina and achieves micro-scale depth targeting accuracy, showing its promising possibility for clinical application.

In the second part, we propose and demonstrate selective retina therapy (SRT) monitoring and temperature estimation based on speckle variance OCT (svOCT) for dosimetry control. SRT is an effective laser treatment method for retinal diseases associated with a degradation of the retinal pigment epithelium (RPE). The SRT selectively targets the RPE, so it reduces negative side effects and facilitates healing of the induced retinal lesions. However, the selection of proper laser energy is challenging because of ophthalmoscopically invisible lesions in the RPE and variance in melanin concentration between patients and even between regions within an eye. SvOCT quantifies speckle pattern variation caused by moving particles or structural changes in biological tissues. SvOCT images were calculated as interframe intensity variance of the sequence, and they show abrupt speckle variance change induced by laser pulse irradiation. We find that svOCT peak values have a reliable correlation with the degree of retinal lesion formation. The temperature at the neural retina and RPE is estimated from the svOCT peak values using numerically calculated temperature, which is consistent with the observed lesion creation.

Committee Members

  • Jin U. Kang, Department of Electrical and Computer Engineering
  • Israel Gannot, Department of Electrical and Computer Engineering
  • Mark Foster, Department of Electrical and Computer Engineering
Jul
6
Tue
Thesis Proposal: Honghua Guan
Jul 6 @ 12:30 pm
Thesis Proposal: Honghua Guan

Note: This is a virtual presentation. Here is the link for where the presentation will be taking place.

Title: High-throughput Optical Explorer in Freely-behaving Rodents

Abstract: One critical goal for neuroscience is to explore the mechanisms underlying neuronal information processing. A suitable brain imaging tool is of great significance to be capable of recording clear neuronal signals over prolonged periods. Among different imaging modalities, multiphoton microscopy becomes the choice for in vivo brain applications owing to its subcellular resolution, optical sectioning and deep penetration. The current experimental routine, however, requires head-fixation of animals during data acquisition. This configuration will inevitably introduce unwanted stress and limit many behavior studies such as social interaction. The scanning two-photon fiberscope is a promising technical direction to bridge this gap. Benefiting from its ultra-compact design and light-weight, it is an ideal optical brain imaging modality to assess dynamic neuronal activities in freely-behaving rodents with subcellular resolution. One significant challenge with the compact scanning two-photon fiberscope is its suboptimal imaging throughput due to the limited choices of miniature optomechanical components.

In this project, we present a compact multicolor two-photon fiberscope platform. We achieve three-wavelength excitation by synchronizing the pulse trains from a femtosecond OPO and its pump. The imaging results demonstrate that we can excite several different fluorescent proteins simultaneously with an optimal excitation efficiency. In addition, we propose a deep neural network (DNN) based solution that significantly improves the imaging frame rate with minimal loss in image quality. This innovation enables 10-fold speed enhancement for the scanning two-photon fiberscope, making it feasible to perform video-rate (26 fps) two-photon imaging in freely-moving mice with excellent imaging resolution and SNR that were previously not possible.

Committee Members

  • Xingde Li, Department of Biomedical Engineering
  • Mark Foster, Department of Electrical and Computer Engineering
  • Jing U. Kang, Department of Electrical and Computer Engineering
  • Israel Gannot, Department of Electrical and Computer Engineering
  • Hui Lu, Department of Pharmacology and Physiology, George Washington University
Aug
9
Mon
Dissertation Defense: Debojyoti Biswas
Aug 9 @ 10:00 am
Dissertation Defense: Debojyoti Biswas

Note: This is a virtual presentation. Here is the link for where the presentation will be taking place.

Title: Stochastic Models of Chemotaxing Signaling Processes

Abstract: Stochasticity is ubiquitous in all processes. Its contribution in shaping the output response is not only restricted to systems involving entities with low copy numbers. Intrinsic fluctuations can also affect systems in which the interacting species are present in abundance. Chemotaxis, the migration of cells towards chemical cues, is one such example. Chemotaxis is a fundamental process that is behind a wide range of biological events, ranging from the innate immune response of organisms to cancer metastasis. In this dissertation, we study the role that stochastic fluctuations play in the regulatory mechanism that regulates chemotaxis in the social amoeba Dictyostelium discoideum. It has been argued theoretically and shown experimentally that stochastically driven threshold crossings of an underlying excitable system, lead to the protrusions that enable amoeboid cells to move. To date, however, there has been no good computational model that accurately accounts for the effects of noise, as most models merely inject noise extraneously to deterministic models leading to stochastic differential equations. In contrast, in this study, we employ an entirely different paradigm to account for the noise effects, based on the reaction-diffusion master equation. Using a modular approach and a three-dimensional description of the cell model with specific subdomains attributed to the cell membrane and cortex, we develop a detailed model of the receptor-mediated regulation of the signal transduction excitable network (STEN), which has been shown to drive actin dynamics. Using this model, we recreate the patterns of wave propagation seen in both front- and back-side markers that are seen experimentally. Moreover, we recreate various perturbations. Our model provides further support for the biased excitable network hypothesis that posits that directed motion occurs from a spatially biased regulation of the threshold for activation of an excitable network.

Here we also consider another aspect of the chemotactic response. While front- and back-markers redistribute in response to chemoattractant gradients, over time, this spatial heterogeneity becomes established and can exist even when the external chemoattractant gradient is removed. We refer to this persistent segregation of the cell into back and front regions as polarity. In this dissertation, we study various methods by which polarity can be established. For example, we consider the role of vesicular trafficking as a means of bringing back-markers from the front to the rear of the cell. Then, we study how BAR-domain proteins that are sensitive to membrane curvature, can amplify small shape heterogeneities leading to cell polarization. Finally, we develop computational models that describe a novel framework by which polarity can be established and perturbed through the alteration of the charge distribution on the inner leaf of the cell membrane.

Committee Members

  • Pablo A. Iglesias, Department of Electrical and Computer Engineering
  • Noah J . Cowan, Department of Mechanical Engineering
  • Enrique Mallada, Department of Electrical and Computer Engineering
  • Peter N. Devreotes, Department of Cell Biology
Aug
12
Thu
Dissertation Defense: Yufan He
Aug 12 @ 1:00 pm
Dissertation Defense: Yufan He

Note: This is a virtual presentation. Here is the link for where the presentation will be taking place.

Title: Retinal OCT Image Analysis Using Deep Learning

Abstract: Optical coherence tomography (OCT) is a noninvasive imaging modality which uses low coherence light waves to take cross-sectional images of optical scattering media. OCT has been widely used in diagnosing retinal and neural diseases by imaging the human retina. The thickness of retinal layers are important biomarkers for neurological diseases like multiple sclerosis (MS). The peripapillary retinal nerve fiber layer (pRNFL) and ganglion cell plus inner plexiform layer (GCIP) thickness can be used to assess global disease progression of MS patients. Automated OCT image analysis tools are critical for quantitatively monitoring disease progression and explore biomarkers. With the development of more powerful computational resources, deep learning based methods have achieved much better performance in accuracy, speed, and algorithm flexibility for many image analysis tasks. However, without task-specific modifications, these emerging deep learning methods are not satisfactory if directly applied to tasks like retinal layer segmentation.

In this thesis, we present a set of novel deep learning based methods for OCT image analysis. Specifically, we focus on automated retinal layer segmentation from macular OCT images. A first problem we address is that existing deep learning methods do not incorporate explicit anatomical rules and cannot guarantee the layer segmentation hierarchy (pixels of the upper layers should have no overlap or gap with pixels of layers beneath it). To solve this, we developed an efficient fully convolutional network to generate structured layer surfaces with correct topology that is also able to perform retinal lesion (cysts or edema) segmentation. A second problem we addressed is that the segmentation uncertainty reduces the sensitivity of detecting mild retinal changes in MS patients overtime. To solve this, we developed a longitudinal deep learning pipeline that considers both inter-slice and longitudinal segmentation priors to achieve a more consistent segmentation for monitoring patient-specific retinal changes. A third problem we addressed is that the performance of the deep learning models will degrade when test data is generated from different scanners (domain shift). We address this problem by developing a novel test-time domain adaptation method. Different than existing solutions, our model can dynamically adapt to each test subject during inference without time-consuming retraining. Our deep networks achieved state-of-the-art segmentation accuracy, speed, and flexibility comparing to the existing methods.

Committee Members

  • Jerry Prince, Department of Electrical and Computer Engineering
  • Archana Venkataraman, Department of Electrical and Computer Engineering
  • Vishal Patel, Department of Electrical and Computer Engineering
Aug
25
Wed
Dissertation Defense: Honghua Guan
Aug 25 @ 1:00 pm
Dissertation Defense: Honghua Guan

Note: This is a virtual presentation. Here is the link for where the presentation will be taking place.

Title: High-throughput Optical Explorer in Freely-behaving Rodents

Abstract: Optical brain imaging is one of the most important branches of the neuroimaging. It has seen thirty years of intense development. To monitor neuronal activities in vivo, calcium imaging and sensing technique is widely used for various neuroscience investigations by measuring the calcium (Ca2+) status of an isolated cell or a population of cells. Benefiting from different types of genetically encoded calcium indicators (GECI), especially the GCaMP family, optical calcium imaging enables to monitor the electrical activity in hundreds of neurons in cell culture or in living animals, which has made it possible to elucidate the function of neuronal circuits at fine spatial resolution.

Among different optical brain imaging tools, multiphoton microscopy, owing to its depth-resolving ability, confined excitation volume and deep imaging penetration, has become the standard choice for noninvasive in vivo brain imaging. However, the current experimental routine requires head-fixation of animals during data acquisition. This configuration will inevitably introduce unwanted stress and limit many behavioral studies such as reward/punishment training, memory, and social interaction. The scanning two-photon fiberscope described in this thesis is a promising technical direction to bridge this gap. Owing to the ultra-compact design and light weight, it is an ideal optical brain imaging modality to assess dynamic neuronal activities in freely-behaving rodents with subcellular resolution. One significant challenge with the compact scanning two-photon fiberscope is its suboptimal imaging throughput due to the limited choices of miniature optomechanical components.

This dissertation reports our efforts in improving the throughput of two-photon fiberscope system from different perspectives, which includes introducing multiple-wavelength excitation for simultaneous multicolor imaging, increasing imaging speed, enlarging field of view (FOV) and so on. We also discuss our contributions on animal model preparation protocols for in vivo imaging in freely-behaving mice.

The improvement of system throughput enables us to explore many new applications that were previously impractical or impossible. We first report a compact multicolor two-photon fiberscope platform. We used two coherent pulsed outputs (the pump and the Stokes beams) from an optical parametric oscillator (OPO). By temporal and spatial overlapping, we could synchronize the two coherent pulses and generate the third virtual wavelength. These three wavelengths, which cover a large range from 750 nm to 1200 nm, are suitable for many fluorescent proteins and calcium indicators that are commonly used in neuroscience studies. This method shows more benefits in practice (e.g., reasonable cost, integrated system). The imaging results acquired from “Brainbow” mouse model demonstrate that we can excite several different fluorescent proteins simultaneously with an optimal excitation efficiency.

In addition, we proposed a deep-learning (DL) based solution that can significantly improve the imaging frame rate with minimal loss in image quality. A two-step learning transfer strategy was introduced to generate appropriate training datasets for improving the quality (signal-to-noise ratio and spatial imaging resolution) of high-speed in vivo images. The method allowed for a more than 10-fold increase in imaging speed (from ~2.0 fps to ~26 fps) while maintaining a high SNR and imaging resolution. This new DL-assisted two-photon fiberscope opens up new avenues for studying and understanding the neural basis of behaviors.

Committee Members

  • Xingde Li, Department of Biomedical Engineering
  • Mark Foster, Department of Electrical and Computer Engineering
  • Jing U. Kang, Department of Electrical and Computer Engineering
  • Israel Gannot, Department of Electrical and Computer Engineering
  • Hui Lu, Department of Pharmacology and Physiology, George Washington University
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