Secondary Appointments

Jung Soo Suk
Secondary Appointment

Primary Affiliation: Assistant Professor, Johns Hopkins University School of Medicine

https://www.hopkinsmedicine.org/profiles/results/directory/profile/10002107/jung-soo-suk

jsuk@jhmi.edu

Netz Arroyo
Secondary Appointment

netzarroyo@jhmi.edu

Elia Duh
Secondary Appointment

Primary Affiliation:  Professor, Johns Hopkins University School of Medicine, Ophthalmology

https://www.hopkinsmedicine.org/profiles/results/directory/profile/0008957/elia-duh

eduh@jhmi.edu

Lakshmi Santhanam
Secondary Appointment

Primary Affiliation: Associate Professor of Anesthesiology and Critical Care Medicine

https://www.hopkinsmedicine.org/profiles/results/directory/profile/7872994/lakshmi-santhanam

lsantha1@jhmi.edu

H. Benjamin Larman
Secondary Appointment

PhD, Harvard-MIT Division of Health Science and Technology

Primary Affiliation: Assistant Professor, Johns Hopkins School of Medicine, Immunopathology

Lab

 

hlarman1@jhmi.edu

Warren Grayson
Secondary Appointment

Primary Affiliation: Assistant professor of biomedical engineering at Johns Hopkins University School of Medicine

Dr. Warren Grayson is an Associate Professor of Biomedical Engineering and Material Sciences & Engineering at Johns Hopkins University, a founding member of the Translational Tissue Engineering Center, and an affiliate member of the Institute for NanoBioTechnology. He received his secondary appointment in the Department of Chemical & Biomolecular Engineering in 2020. His research interests focus on the stem cell-based regeneration of musculoskeletal tissues. He is a pioneer in the field of 3D-printing porous, biodegradable scaffolds and bioreactor design for personalized, craniofacial bone regeneration. His work on engineering anatomically shaped bone grafts has received coverage in the New York Times, BBC, and Science Translational Medicine among others, and has spawned a start-up company. Dr. Grayson’s laboratory is currently studying how to regenerate functional skeletal muscle following volumetric tissue loss using advanced biomaterials, bioreactors, and stem cells.  

He has been recognized by the Maryland Academy of Sciences with the Allan C. Davis Medal for the Outstanding Young Engineer. He has also received the Career Development Award from the Orthopaedic Research Society, the Career Enhancement Award from the American Society for Bone and Mineral Research, the Young Investigator Award from the Tissue Engineering and Regenerative Medicine International Society, and the prestigious Early Faculty CAREER Award from the National Science Foundation. He was recently elected as a fellow of the American Institute for Medical and Biomedical Engineering and was invited by the National Academy of Medicine for their symposium for Emerging Leaders in Health and Medicine. He holds patents in biomaterial and bioreactor design and has authored over 80 original and review articles and book chapters.

Prof. Grayson earned his BS in Chemical & Process Engineering at the University of the West Indies in Trinidad, WI in 1998. He obtained his Ph.D. in Biomedical Engineering from the Florida State University in 2005. He did his post-doctoral studies at Columbia University in the city of New York.

wgrayson@jhmi.edu

Rigoberto Hernandez
Professor

Primary Affiliation: Gompf Family Professor, Department of Chemistry, Johns Hopkins University

https://chemistry.jhu.edu/directory/rigoberto-hernandez/

 

Ph.D. Chemistry, University of California, Berkeley (’93)

BSE Chemical Engineering, Princeton University (’89)

 

Research Interests: Soft Matter Nanomaterials, Nano-Bio Interface, Nonequilibrium Dynamics, Multiscale Dynamics, Reaction Rate Theory

r.hernandez@jhu.edu

Anne Le
Associate Professor

Primary Affiliation: Associate Professor of Pathology and Oncology

Lab website: http://pathology.jhu.edu/lelab/index.cfm

annele@jhmi.edu

Margaret Johnson
Assistant Professor

Margaret Johnson is an Assistant Professor in the Department of Biophysics, which she joined in 2013. She received her B.S. in Applied Math from Columbia University and her PhD in Bioengineering from UC Berkeley. She completed her postdoc in the Lab of Chemical Physics at the NIH.  Her multidisciplinary research group studies dynamical systems in biology. Her group addresses how macromolecular self-assembly is controlled to occur at the right place and time, with focus on endocytosis, viral exit, and transcriptional regulation. Group members are interested both in principles and design of self-assembly, as well as biological function in cells. They develop and apply theory, modeling, and simulations to these problems, with frequent collaboration with experimentalists. Margaret has received an NIH Pathway to Independence Award, an NSF CAREER Award, and an NIH MIRA Award.

 

Curriculum Vitae

 

Research

Cells are complex dynamical systems, operating out-of-equilibrium. How do the many components within the cell self-organize and assemble at the right place for proper biological and mechanical function? To study these complex systems, we simulate networks of many interacting components, and construct simplified models that are amenable to analytical theory. We use and develop modeling techniques from physics, chemistry, and engineering, including molecular models for thermodynamics, reaction-diffusion models for stochastic dynamics in cells, and continuum models for coupling to mechanics. We are interested in principles and design of optimal self-assembly, as well as the development of predictive models for cell biological processes. The dynamics of self-assembly in the cell are challenging to simulate due to their dependence on fast molecular motion and slow collective dynamics, along with coupling to energy-consuming reactions. The reaction-diffusion methods developed in our lab enable simulations inaccessible to existing software tools, with broad applications in cell biology and engineering design.

Two fascinating examples of macromolecular self-assembly in cell biology are clathrin-mediated endocytosis (CME) and virion formation during HIV infection. In both cases, proteins assemble into a spherical structure that is required to perform the work to remodel the membrane into a vesicle, either for transport in (CME) or out (viral budding) of the cell.  Understanding the control and spatio-temporal dynamics of these assembly problems coupled to membrane bending is a shared theme of both our research projects that allows us to learn from each other and apply similar methodologies.

Clathrin-mediated endocytosis is occurring persistently within our cells, performing transport of essential nutrients such as iron into our cells, and clearing receptors at our neural synapse. It involves coordinated assembly of dozens of different protein components, and viral infection or neurological disorders can result from its dysfunction. Our research has shown how localization to the membrane can dramatically enhance assembly of even very weakly interacting partners via dimensionality reduction from 3D to 2D, which effectively concentrates components and promotes binding. At the systems level, we have quantified how the stoichiometry of the network of protein and lipid components involved in CME can sensitively tune the speed or success of vesicle formation, highlighting again the central role of membrane localization in triggering protein assembly. Ongoing research in our lab is focused on predicting the timescales of binding in and on the 2D membrane, mechanical feedback between membrane binding and bending, and timescales of receptor and cargo uptake. We are broadly interested in how key features of dimensionality and component stoichiometry control assembly in CME, virion formation, and other cellular pathways.

 

Group/Lab Website

margaret.johnson@jhu.edu

Patrick Breysse
Secondary Appointment

Primary Affiliation: Professor, Johns Hopkins University School of Medicine, Division of Environmental Health Engineering

pbreyss1@jhu.edu

Jennifer Elisseeff
Secondary Appointment

Primary Affiliations: Professor, Johns Hopkins University School of Medicine, Department of Ophthalmology
Director of the Translational Tissue Engineering Center.

jhe@jhu.edu

Laura Ensign-Hodges
Secondary Appointment

Primary Affiliation: Assistant Professor, Johns Hopkins University School of Medicine, Ophthalmology

https://www.hopkinsmedicine.org/profiles/results/directory/profile/7704994/laura-ensign

lensign1@jhu.edu

Jonah Erlebacher
Secondary Appointment

Primary Affiliation: Professor & Chair, Johns Hopkins University Whiting School of Engineering, Materials Science and Engineering

https://engineering.jhu.edu/materials/faculty/Jonah-Erlebacher/

jonah.erlebacher@jhu.edu

Daniele Gilkes
Secondary Appointment

Primary affiliation:  Assistant Professor, Johns Hopkins University School of Medicine, Department of Oncology  Breast and Ovarian Cancer

dgilkes1@jhu.edu

Justin Hanes
Secondary Appointment

Primary Affiliation: Lewis J. Ort Professor of Ophthalmology, Johns Hopkins University School of Medicine, Ophthalmology

hanes@jhmi.edu

John Isaacs
Secondary Appointment

Primary Affiliation: Professor, Johns Hopkins University, School of Medicine

isaacjo@jhmi.edu

Kenneth Pienta
Secondary Appointment

Primary Affiliation: Professor, Johns Hopkins University School of Medicine, Urology Research

kpienta1@jhmi.edu

Martin Pomper
Secondary Appointment

Primary Affiliation: Professor, Johns Hopkins University School of Medicine, Radiology

mpomper@jhmi.edu

Kannan Rangaramanujam
Secondary Appointment

Primary Affiliation: Professor, Johns Hopkins University School of Medicine, Ophthalmology

krangar1@jhmi.edu

Doug Robinson
Secondary Appointment

Primary Affiliation: Professor, Johns Hopkins University School of Medicine, Cell Biology

dnr@jhmi.edu

Hui Zhang
Secondary Appointment

Primary Affiliation: Professor, Johns Hopkins University School of Medicine, Department of Pathology

https://www.hopkinsmedicine.org/profiles/results/directory/profile/7844241/hui-zhang

huizhang@jhu.edu


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