Secondary Appointments
Netz Arroyo
Secondary Appointment
Primary affiliation: Pharmacology and Molecular Sciences
https://www.hopkinsmedicine.org/pharmacology_molecular_sciences/faculty/bios/arroyo.html
Elia Duh
Secondary Appointment
Primary Affiliation: Professor, Johns Hopkins University School of Medicine, Ophthalmology
https://www.hopkinsmedicine.org/profiles/results/directory/profile/0008957/elia-duh
Lakshmi Santhanam
Secondary Appointment
Primary Affiliation: Associate Professor of Anesthesiology and Critical Care Medicine
https://www.hopkinsmedicine.org/profiles/results/directory/profile/7872994/lakshmi-santhanam
H. Benjamin Larman
Secondary Appointment
PhD, Harvard-MIT Division of Health Science and Technology
Primary Affiliation: Assistant Professor, Johns Hopkins School of Medicine, Immunopathology
Warren Grayson
Secondary Appointment
Primary Affiliation: Assistant professor of biomedical engineering at Johns Hopkins University School of Medicine
Dr. Warren Grayson is an Associate Professor of Biomedical Engineering and Material Sciences & Engineering at Johns Hopkins University, a founding member of the Translational Tissue Engineering Center, and an affiliate member of the Institute for NanoBioTechnology. He received his secondary appointment in the Department of Chemical & Biomolecular Engineering in 2020. His research interests focus on the stem cell-based regeneration of musculoskeletal tissues. He is a pioneer in the field of 3D-printing porous, biodegradable scaffolds and bioreactor design for personalized, craniofacial bone regeneration. His work on engineering anatomically shaped bone grafts has received coverage in the New York Times, BBC, and Science Translational Medicine among others, and has spawned a start-up company. Dr. Grayson’s laboratory is currently studying how to regenerate functional skeletal muscle following volumetric tissue loss using advanced biomaterials, bioreactors, and stem cells.
He has been recognized by the Maryland Academy of Sciences with the Allan C. Davis Medal for the Outstanding Young Engineer. He has also received the Career Development Award from the Orthopaedic Research Society, the Career Enhancement Award from the American Society for Bone and Mineral Research, the Young Investigator Award from the Tissue Engineering and Regenerative Medicine International Society, and the prestigious Early Faculty CAREER Award from the National Science Foundation. He was recently elected as a fellow of the American Institute for Medical and Biomedical Engineering and was invited by the National Academy of Medicine for their symposium for Emerging Leaders in Health and Medicine. He holds patents in biomaterial and bioreactor design and has authored over 80 original and review articles and book chapters.
Prof. Grayson earned his BS in Chemical & Process Engineering at the University of the West Indies in Trinidad, WI in 1998. He obtained his Ph.D. in Biomedical Engineering from the Florida State University in 2005. He did his post-doctoral studies at Columbia University in the city of New York.
Rigoberto Hernandez
Professor
Primary Affiliation: Gompf Family Professor, Department of Chemistry, Johns Hopkins University
https://chemistry.jhu.edu/directory/rigoberto-hernandez/
Ph.D. Chemistry, University of California, Berkeley (’93)
BSE Chemical Engineering, Princeton University (’89)
Research Interests: Soft Matter Nanomaterials, Nano-Bio Interface, Nonequilibrium Dynamics, Multiscale Dynamics, Reaction Rate Theory
Anne Le
Associate Professor
Primary Affiliation: Associate Professor of Pathology and Oncology
Lab website: http://pathology.jhu.edu/lelab/index.cfm
Margaret Johnson
Assistant Professor
Margaret Johnson is an Assistant Professor in the Department of Biophysics, which she joined in 2013. She received her B.S. in Applied Math from Columbia University and her PhD in Bioengineering from UC Berkeley. She completed her postdoc in the Lab of Chemical Physics at the NIH. Her multidisciplinary research group studies dynamical systems in biology. Her group addresses how macromolecular self-assembly is controlled to occur at the right place and time, with focus on endocytosis, viral exit, and transcriptional regulation. Group members are interested both in principles and design of self-assembly, as well as biological function in cells. They develop and apply theory, modeling, and simulations to these problems, with frequent collaboration with experimentalists. Margaret has received an NIH Pathway to Independence Award, an NSF CAREER Award, and an NIH MIRA Award.
Research
Cells are complex dynamical systems, operating out-of-equilibrium. How do the many components within the cell self-organize and assemble at the right place for proper biological and mechanical function? To study these complex systems, we simulate networks of many interacting components, and construct simplified models that are amenable to analytical theory. We use and develop modeling techniques from physics, chemistry, and engineering, including molecular models for thermodynamics, reaction-diffusion models for stochastic dynamics in cells, and continuum models for coupling to mechanics. We are interested in principles and design of optimal self-assembly, as well as the development of predictive models for cell biological processes. The dynamics of self-assembly in the cell are challenging to simulate due to their dependence on fast molecular motion and slow collective dynamics, along with coupling to energy-consuming reactions. The reaction-diffusion methods developed in our lab enable simulations inaccessible to existing software tools, with broad applications in cell biology and engineering design.
Two fascinating examples of macromolecular self-assembly in cell biology are clathrin-mediated endocytosis (CME) and virion formation during HIV infection. In both cases, proteins assemble into a spherical structure that is required to perform the work to remodel the membrane into a vesicle, either for transport in (CME) or out (viral budding) of the cell. Understanding the control and spatio-temporal dynamics of these assembly problems coupled to membrane bending is a shared theme of both our research projects that allows us to learn from each other and apply similar methodologies.
Clathrin-mediated endocytosis is occurring persistently within our cells, performing transport of essential nutrients such as iron into our cells, and clearing receptors at our neural synapse. It involves coordinated assembly of dozens of different protein components, and viral infection or neurological disorders can result from its dysfunction. Our research has shown how localization to the membrane can dramatically enhance assembly of even very weakly interacting partners via dimensionality reduction from 3D to 2D, which effectively concentrates components and promotes binding. At the systems level, we have quantified how the stoichiometry of the network of protein and lipid components involved in CME can sensitively tune the speed or success of vesicle formation, highlighting again the central role of membrane localization in triggering protein assembly. Ongoing research in our lab is focused on predicting the timescales of binding in and on the 2D membrane, mechanical feedback between membrane binding and bending, and timescales of receptor and cargo uptake. We are broadly interested in how key features of dimensionality and component stoichiometry control assembly in CME, virion formation, and other cellular pathways.
Jung Soo Suk
Secondary Appointment
Primary Affiliation: Assistant Professor, Johns Hopkins University School of Medicine
https://www.hopkinsmedicine.org/profiles/results/directory/profile/10002107/jung-soo-suk
Patrick Breysse
Secondary Appointment
Primary Affiliation: Professor, Johns Hopkins University School of Medicine, Division of Environmental Health Engineering
Jennifer Elisseeff
Secondary Appointment
Primary Affiliations: Professor, Johns Hopkins University School of Medicine, Department of Ophthalmology
Director of the Translational Tissue Engineering Center.
Laura Ensign-Hodges
Secondary Appointment
Primary Affiliation: Assistant Professor, Johns Hopkins University School of Medicine, Ophthalmology
https://www.hopkinsmedicine.org/profiles/results/directory/profile/7704994/laura-ensign
Jonah Erlebacher
Secondary Appointment
Primary Affiliation: Professor & Chair, Johns Hopkins University Whiting School of Engineering, Materials Science and Engineering
https://engineering.jhu.edu/materials/faculty/Jonah-Erlebacher/
Daniele Gilkes
Secondary Appointment
Primary affiliation: Assistant Professor, Johns Hopkins University School of Medicine, Department of Oncology Breast and Ovarian Cancer
Justin Hanes
Secondary Appointment
Primary Affiliation: Lewis J. Ort Professor of Ophthalmology, Johns Hopkins University School of Medicine, Ophthalmology
John Isaacs
Secondary Appointment
Primary Affiliation: Professor, Johns Hopkins University, School of Medicine
Kenneth Pienta
Secondary Appointment
Primary Affiliation: Professor, Johns Hopkins University School of Medicine, Urology Research
Martin Pomper
Secondary Appointment
Primary Affiliation: Professor, Johns Hopkins University School of Medicine, Radiology
Kannan Rangaramanujam
Secondary Appointment
Primary Affiliation: Professor, Johns Hopkins University School of Medicine, Ophthalmology
Doug Robinson
Secondary Appointment
Primary Affiliation: Professor, Johns Hopkins University School of Medicine, Cell Biology
Hui Zhang
Secondary Appointment
Primary Affiliation: Professor, Johns Hopkins University School of Medicine, Department of Pathology
https://www.hopkinsmedicine.org/profiles/results/directory/profile/7844241/hui-zhang
