Daniel J. Leahy
Method developed in our lab for measuring membrane protein interactions.
Cellular responses to stimuli can be understood and predicted based on quantitative knowledge of interaction strengths between proteins involved in signaling. Towards this goal, we are developing experimental methods that yield a quantitative description of protein interactions in cellular membranes, and is striving to achieve a comprehensive understanding of the communication between the cell and its environment, as mediated by the receptors on the cell surface.
In one current project, we are using Forster Resonance Energy Transfer (FRET) to probe the effects of the activating pathogenic mutations on FGFR3 dimerization. Changes in the dimerization propensity are being measured in the plasma membrane and in plasma membrane derived vesicles.